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hygromycin b raji cells  (ATCC)


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    ATCC hygromycin b raji cells
    Hygromycin B Raji Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 3500 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hygromycin b raji cells/product/ATCC
    Average 99 stars, based on 3500 article reviews
    hygromycin b raji cells - by Bioz Stars, 2026-06
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    ( A ) Analysis of ChIP-seq data of p300 (black), histone (blue), NFATc1 (green), and in situ Hi-C data at the IL10 locus using public datasets ( GSE32465 , GSE29611 , and GSE63525 ) of GM12878 human immortalized B cells. ( B to E <t>)</t> <t>Raji</t> <t>B</t> cells were electroporated with vectors expressing Cas9 and sgRNAs targeting NFAT-binding motifs in IL10 CNS-12 or mock vectors. (B) Schematic diagram of the NFAT-binding motifs in IL10 CNS-12, identified with rVISTA (Transfac matrices, similarity score of 0.85). Red arrows indicate sgRNA targeting sites. (C) qRT-PCR analysis of IL10 mRNA in electroporated human Raji B cells, expressed as fold change relative to unstimulated cells. [(D) and (E)] Representative flow cytometry plots (D) and frequency (E) of IL-10 + cells in Raji B cells ( n = 3 for mock, sgRNA 1, and sgRNA 2). A FMO control for IL-10 is shown in (D). ( F ) IL-10 concentrations in culture supernatants of Raji B cells measured at 4 and 24 hours ( n = 3 for mock, sgRNA 1, and sgRNA 2). Data are pooled from three independent experiments [(C), (E), and (F)]. Data are presented as means ± SEM. Statistical analysis was performed using a two-tailed unpaired Student’s t test [(C), (E), and (F)]: * P < 0.05, ** P < 0.01, and **** P < 0.0001.
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    ( A ) Analysis of ChIP-seq data of p300 (black), histone (blue), NFATc1 (green), and in situ Hi-C data at the IL10 locus using public datasets ( GSE32465 , GSE29611 , and GSE63525 ) of GM12878 human immortalized B cells. ( B to E <t>)</t> <t>Raji</t> <t>B</t> cells were electroporated with vectors expressing Cas9 and sgRNAs targeting NFAT-binding motifs in IL10 CNS-12 or mock vectors. (B) Schematic diagram of the NFAT-binding motifs in IL10 CNS-12, identified with rVISTA (Transfac matrices, similarity score of 0.85). Red arrows indicate sgRNA targeting sites. (C) qRT-PCR analysis of IL10 mRNA in electroporated human Raji B cells, expressed as fold change relative to unstimulated cells. [(D) and (E)] Representative flow cytometry plots (D) and frequency (E) of IL-10 + cells in Raji B cells ( n = 3 for mock, sgRNA 1, and sgRNA 2). A FMO control for IL-10 is shown in (D). ( F ) IL-10 concentrations in culture supernatants of Raji B cells measured at 4 and 24 hours ( n = 3 for mock, sgRNA 1, and sgRNA 2). Data are pooled from three independent experiments [(C), (E), and (F)]. Data are presented as means ± SEM. Statistical analysis was performed using a two-tailed unpaired Student’s t test [(C), (E), and (F)]: * P < 0.05, ** P < 0.01, and **** P < 0.0001.
    B Cells Raji, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ( A ) Analysis of ChIP-seq data of p300 (black), histone (blue), NFATc1 (green), and in situ Hi-C data at the IL10 locus using public datasets ( GSE32465 , GSE29611 , and GSE63525 ) of GM12878 human immortalized B cells. ( B to E <t>)</t> <t>Raji</t> <t>B</t> cells were electroporated with vectors expressing Cas9 and sgRNAs targeting NFAT-binding motifs in IL10 CNS-12 or mock vectors. (B) Schematic diagram of the NFAT-binding motifs in IL10 CNS-12, identified with rVISTA (Transfac matrices, similarity score of 0.85). Red arrows indicate sgRNA targeting sites. (C) qRT-PCR analysis of IL10 mRNA in electroporated human Raji B cells, expressed as fold change relative to unstimulated cells. [(D) and (E)] Representative flow cytometry plots (D) and frequency (E) of IL-10 + cells in Raji B cells ( n = 3 for mock, sgRNA 1, and sgRNA 2). A FMO control for IL-10 is shown in (D). ( F ) IL-10 concentrations in culture supernatants of Raji B cells measured at 4 and 24 hours ( n = 3 for mock, sgRNA 1, and sgRNA 2). Data are pooled from three independent experiments [(C), (E), and (F)]. Data are presented as means ± SEM. Statistical analysis was performed using a two-tailed unpaired Student’s t test [(C), (E), and (F)]: * P < 0.05, ** P < 0.01, and **** P < 0.0001.
    Cell Lines Raji, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ( A ) Analysis of ChIP-seq data of p300 (black), histone (blue), NFATc1 (green), and in situ Hi-C data at the IL10 locus using public datasets ( GSE32465 , GSE29611 , and GSE63525 ) of GM12878 human immortalized B cells. ( B to E ) Raji B cells were electroporated with vectors expressing Cas9 and sgRNAs targeting NFAT-binding motifs in IL10 CNS-12 or mock vectors. (B) Schematic diagram of the NFAT-binding motifs in IL10 CNS-12, identified with rVISTA (Transfac matrices, similarity score of 0.85). Red arrows indicate sgRNA targeting sites. (C) qRT-PCR analysis of IL10 mRNA in electroporated human Raji B cells, expressed as fold change relative to unstimulated cells. [(D) and (E)] Representative flow cytometry plots (D) and frequency (E) of IL-10 + cells in Raji B cells ( n = 3 for mock, sgRNA 1, and sgRNA 2). A FMO control for IL-10 is shown in (D). ( F ) IL-10 concentrations in culture supernatants of Raji B cells measured at 4 and 24 hours ( n = 3 for mock, sgRNA 1, and sgRNA 2). Data are pooled from three independent experiments [(C), (E), and (F)]. Data are presented as means ± SEM. Statistical analysis was performed using a two-tailed unpaired Student’s t test [(C), (E), and (F)]: * P < 0.05, ** P < 0.01, and **** P < 0.0001.

    Journal: Science Advances

    Article Title: Conserved noncoding sequence-9 regulates NFATc1-mediated IL-10 expression in B cells to control inflammatory responses

    doi: 10.1126/sciadv.aec7779

    Figure Lengend Snippet: ( A ) Analysis of ChIP-seq data of p300 (black), histone (blue), NFATc1 (green), and in situ Hi-C data at the IL10 locus using public datasets ( GSE32465 , GSE29611 , and GSE63525 ) of GM12878 human immortalized B cells. ( B to E ) Raji B cells were electroporated with vectors expressing Cas9 and sgRNAs targeting NFAT-binding motifs in IL10 CNS-12 or mock vectors. (B) Schematic diagram of the NFAT-binding motifs in IL10 CNS-12, identified with rVISTA (Transfac matrices, similarity score of 0.85). Red arrows indicate sgRNA targeting sites. (C) qRT-PCR analysis of IL10 mRNA in electroporated human Raji B cells, expressed as fold change relative to unstimulated cells. [(D) and (E)] Representative flow cytometry plots (D) and frequency (E) of IL-10 + cells in Raji B cells ( n = 3 for mock, sgRNA 1, and sgRNA 2). A FMO control for IL-10 is shown in (D). ( F ) IL-10 concentrations in culture supernatants of Raji B cells measured at 4 and 24 hours ( n = 3 for mock, sgRNA 1, and sgRNA 2). Data are pooled from three independent experiments [(C), (E), and (F)]. Data are presented as means ± SEM. Statistical analysis was performed using a two-tailed unpaired Student’s t test [(C), (E), and (F)]: * P < 0.05, ** P < 0.01, and **** P < 0.0001.

    Article Snippet: A20 and Raji B cell lines were obtained from the American Type Culture Collection (MD, USA).

    Techniques: ChIP-sequencing, In Situ, Hi-C, Expressing, Binding Assay, Quantitative RT-PCR, Flow Cytometry, Control, Two Tailed Test